Organic molecules containing nitrate groups are manufactured primarily for explosives or for their pharmacological effects. The contamination of the environment by explosives, especially by nitroesters and nitroaromatics (NACs), is a worldwide environmental problem since enormous amounts of these compounds were produced during World War I and II. Most contaminated sites are located at ammunition factories and other places where these compounds were handled. One of inexpensive approach for cleanup of soil and water contaminated with these propellants is the use phytoremediation.
The aims of work:
Heavy metals and radionuclides represent high stress factor for environment and for human. Their toxicities or radiotoxicities are expressed in many different effects and it can be cause of indigestions, different dermatitis, changes in blood count, damage of fundamental organs (brain, liver, kidney), cancerous processes etc. For majority of cations the strong binding with –SH, –COOH and –NH2 groups is characteristic. Therefore more important is the lowering of toxic metals on the natural level. Phytoremediation is still a nascent technology that seeks to exploit the metabolic capabilities and growth habits of higher plants: delivering a cheap, soft and safe biological treatment that is applicable to specific contaminated sites and wastewaters is a relatively recent focus.
The aims of work:
Study of plant secondary metabolites
Plants produce a huge variety of compounds exploited by pharmaceutical, food and
cosmetic industry. Production of some plant compounds is problematic due to
their low contents in raw material, difficult field cultivation, slow
growing of plants, etc. As an example could be mentioned anticancer drugs
Taxol from Taxus species or Vinblastine and Vincristine from
Catharanthus roseus. Alternative approach to field cultivation or to material collection
from natural sources is cultivation under controlled conditions on solid or
liquid sterile medium where desired products are harvested from a biomass or
a culture medium. We are currently working mainly on in vitro
production of ginsenosides by Panax ginseng root culture.
Different approaches such as culture conditions, medium
composition, and elicitation are studied to increase root growth as well as
to improve production of ginsenosides.
Tests of anti-inflammatory activity of plant extracts and compounds
Among those plants that are already known to contain biologically active compounds
there is still plenty of species waiting for exploring. The main field of
our interest is the screening of extracts and compounds of plant origin to
find new inhibitors of cyclooxygenases, enzymes involved in mammalian
inflammatory processes. We use in vitro enzymatic assay for this
Derivatization of natural compounds
Herbal extracts are often use in modern medicine. We use herbal extract or specific agent from it. Both possibilities have got some advantages and disadvantages. The main advantage is the possibility of chemical characterization and derivation of new forms. Derivatization is defined as an additional chemical modification of efficacious compounds. New analogues haven’t got only different physical properties like solubility or molecular weight but we can also change transport in organism.